Tissue+Culture+3-4

> We are an organization working on tissue culture in plants and studying the results. Tissue Culture in plants is more or less “Cloning” the plant. Using this we can easily conduct tests on plants without damaging the ecosystem of the world. Rather than damaging it we are actually helping it. We are also working on making healthier fruits, vegetables, and herbs for future generations. Who we are Our C.O Carter Vandoren has spent many years studying and refining the science of tissue culture making it much better and more productive. He as been working at Harvard for almost 18 years to perfect this brilliant technology. He is Currently 45 years of age with a degree in biology and bio genetics. His main and primary focus has been trying to produce better healthier herbs, fruits, vegetables, and any other plant organisms for the future generations.
 * J.E.E.M Tissue Culture developments
 * About us

1839: A Man Named Schann proposed the theory that if you have a sterile environment you could make a single plant develop independently.

1885: Wilhelm Roux took out little fragments of a medullar plate from an embryo of a chicken. He then cultured it. He studied it and established the basis for tissue culturing.

1878: A man named Vochting preformed basic experiments. He stated “in every plant fragment even the smallest ones rest the elements from which the whole body can build up.”

1900: 1st attempting to culture a plant in Non-aseptic conditions.

1902: Haberlandt predicted that you could take an embryo of a cell and duplicate a cell.

1934: A scientist by the name of Went discovered that auxin IAA promotes rapid cell growth.

1951: The discovery that cytokinin, and kinetin promote cell division.

1991: Mutation breeding technology. New systems of plant breeding created genetically modified plant cells with (herbicide resistance, insect resistance, virus/disease resistance and transgenic plants).

1994: Tissue culturing work has been completed, trails are successful. Tissue culturing in plants has been commercialized.

2005: The University of Agricultural Sciences Bangolore tried to revive a species of bananas that is on the decline. They used tissue culturing to make more of these bananas. They only take 6 months to produce full size bananas.

Future: If we can perfect the process of tissue culturing foods then we could end famine all around the world by mass-producing fruits and vegetables. This will also make foods grow faster and bigger than they do in the wild.


 * Science behind the technology **

Tissue culture is the technique used to take out cells or tissues (animal or plant), and grow them in a different environment, such as a petri dish or test tube. In this container, there must be a culture medium, which has a gel called agar, as well as nutrients, sugars, vitamins, and hormones at a specific amount which varies from each tissue. After sterilizing the sample, it is placed in the agar gel. The “explant”, or sample, eventually grows into a fully fledged plant. Some of these explants can even be transferred out of their original container, and put into a new one. This will result in even more plants. This is the reason why many thousands of plants can be made in months. When the plantlets are deemed mature enough, they can be put into a regular pot with so il, and then put into a greenhouse. This entire process is done in a specialized laboratory, in a special ventilated and sterile cabinet. Using tissue culture, one tissue can be made into 1,000,000 new plants.

//**Pictures of Tissue Culture**//:

Plant tissue culture of disease-free plant tissue.

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Tissue Culture of A Mushroom

http://www.nos.org/bio12/b6ah38.1.htm

Tissue Culture Plants in Lab Growing

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Hybrid Orchids propagated from tissue culture

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//**Uses Of This Technology Today:**//

Certain techniques such as meristem tip culture can be used to produce clean plant material from a virused stock, such as potatoes and many kinds of soft fruit.

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A large-scale growth of plant cells in liquid culture in bio-reactors for production of valuable compounds, like plant-derived secondary metabolites and recombinant proteins used as biopharmaceuticals

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The essence of aseptic technique is the exclusion of invading microorganisms during Experimental procedures. If sterile tissues are available, then the exclusion of microorganisms is Accomplished by using sterile instruments and culture media concurrently with standard Bacteriological transfer procedures to avoid extraneous contamination
 * Aseptic Technique**

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"**tissue culture**." __Encyclopædia Britannica__. 2010. Encyclopædia Britannica Online. 21 Dec. 2010 < [] >.
 * References**

Reed, David Wm. "Cloning Plants:Tissue Culture." //Youth Adventure Program//. Department of Horticultural Sciences Texas A&M University, n.d. Web. 21 Dec 2010. .

Mather, Roberts, Jennie P., Penelope E. //Introduction to Cell and Tissue Culture: theory and Technique//. New York, N.Y.: Plenum Press, 1998. 239. eBook.

Silverstars, Bluemoon. "Plant and Animal Tissue Culture: Procedures, Benefits, and Limitations." //Biotech Articles//. Biotech Articles, 5 27 2010. Web. 21 Dec 2010. []

. Kumar, B.S. Satish. "UAS-B to produce Nanjangud rasabale plants through tissue culture soon ." //The Hindu//. The Hindu, 2010. Web. 23 Dec 2010. .